P REPARATION OF A NEW MEDIUM FOR IN VITRO CULTURE OF BORRELIA MICROTTI AND BORRELIA PERSICA

نویسندگان

  • FARZAD OREIZI
  • MEHDI ASSMAR
  • MOHAMMAD SOLEIMANI
  • NOORAIR PIYAZAK
  • SEYED HOSSEIN HOSSEINI From the Departments of Parasitology institute 0f Iran, Tehran, l.R. Iran.
  • ZAHRA ZAMANI *From the Departments of Parasitology, and Biochemistry, Pasteur institute 0f Iran, Tehran, l.R. Iran.
چکیده مقاله:

Borrelia microtti and Borrelia persica are two Iranian strains of spirochetes whose vectors are Ornithodorous tholozani and Ornithodorous erraticus which are responsible for relapsing fever. BSK medium has been used for in vitro culture of other strains of borrelia, although the two mentioned strains could not be successfully cultured in it. We have modified BSK medium by using guinea pig and fetal calf serum instead of rabbit serum. It was observed that for in vitro culture of Borrelia persica, guinea pig serum and for Borrelia microtti, fetal calf serum show the best growth.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

p reparation of a new medium for in vitro culture of borrelia microtti and borrelia persica

borrelia microtti and borrelia persica are two iranian strains of spirochetes whose vectors are ornithodorous tholozani and ornithodorous erraticus which are responsible for relapsing fever. bsk medium has been used for in vitro culture of other strains of borrelia, although the two mentioned strains could not be successfully cultured in it. we have modified bsk medium by using guinea pig and f...

متن کامل

Evaluation of a new culture medium for Borrelia burgdorferi.

We evaluated the new MPM medium for the growth of Borrelia burgdorferi. All 18 blood samples from 17 patients with Lyme disease were negative. Growth studies showed that by day 4, most organisms in MPM were not viable. Our results reinforce the use of BSK medium as the primary choice for growing B. burgdorferi.

متن کامل

: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes

هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...

a fundamental study of "histiriographic metafiction", and "literary genres", as introduced in "new historical philosophy", and tracing them in the works of julian barnes.

abstract a fundamental study of “historio-graphic metafiction” and “literary genres”, as introduced in “new historical philosophy”, and tracing them in the works of julian barnes having studied the two novels, the porcupine and arthur & george, by julian barnes, the researcher has applied linda hutcheon’s historio-graphic metafictional theories to them. the thesis is divided into five cha...

15 صفحه اول

evaluation of pcr and xenodiagnosis assays for detection of borrelia persica in soft ticks ornithodoros tholozani

objective: relapsing fever caused by borrelia persica is an acute tick-borne disease which is transmitted by soft ticks of ornithodoros tholozani to human. the disease is reported from middle east and many regions of iran. detection of infection is problematic since the suspected infected ticks should be fed on animal hosts such as guinea pigs and subsequently after 7-14 days, the animal blood ...

متن کامل

In vitro susceptibility testing of four antibiotics against Borrelia burgdorferi: a comparison of results for the three genospecies Borrelia afzelii, Borrelia garinii, and Borrelia burgdorferi sensu stricto.

MICs and minimal bactericidal concentrations (MBCs) were evaluated for the four antibiotics azithromycin, amoxicillin, ceftriaxone, and doxycycline against the three main genospecies of Borrelia burgdorferi sensu lato. In MBC testing, statistically significant differences between the genospecies could be found in 7 out of 12 comparative evaluations (P < 0.05).

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


عنوان ژورنال

دوره 15  شماره 4

صفحات  227- 230

تاریخ انتشار 2002-02

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

کلمات کلیدی

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023